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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which leads to chromosome instability, and thus may lead to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital part in advertising and marketing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two added splice variants were documented but specific nucleotide sequence data is just not but available.
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Blocking peptides are peptides that bind specially towards the goal antibody and block antibody binding. These peptide typically is made up of the epitope regarded through the antibody.
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This guideline outlines more probable optimization of mobile and immunoassay parameters to make sure the best possible outcomes are...
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This information outlines further doable optimization of more info mobile and immunoassay parameters to be sure the very best results are...
Antibodies bound to the blocking peptide no more bind towards the epitope on the focus on protein. By comparing the staining with the blocked antibody compared to the antibody by itself, one can see which staining is precise.
Goal Information and facts Cyclin E belongs for the really conserved cyclin household, whose associates are characterised by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation designs which add to the temporal coordination of every mitotic celebration. Cyclin E kinds a complex with and features being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates at the G1-S phase boundary which is degraded as cells progress by S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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